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KMID : 0380220060390040448
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Journal of Biochemistry and Molecular Biology
2006 Volume.39 No. 4 p.448 ~ p.451
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Effective Chemopreventive Activity of Genistein against Human Breast Cancer Cells
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Shon Yun-Hee
Park Sun-Dong
Nam Kyung-Soo
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Abstract
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Chemopreventive and cytotoxic effect of genistein against human breast cancer cell lines was investigated. Genistein inhibited cell proliferation in estrogen receptor-positive (MCF-7) and estrogen receptor-negative (MDA-MB-231) human breast carcinoma cell lines. Cytochrome P450 (CYP) 1A1-mediated ethoxyresorufin O-deethylase (EROD) activity was inhibited by genistein in a concentrationdependent manner. Genistein significantly inhibited 12-Otetradecanoylphorbol-13-acetate (TPA)-induced cyclooxygenase-2 activity and protein expression at the concentrations of 10 (p < 0.05), 25 (p < 0.05) and 50 mM (p < 0.01). In addition, ornithine decarboxylase (ODC) activity was reduced to 53.8 % of the control after 6 h treatment with 50 mM genistein in MCF-7 breast cancer cells. These results suggest that genistein could be of therapeutic value in preventing human breast cancer.
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KEYWORD
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Breast cancer, Cyclooxygenase-2, Cytochrome P450 1A1, Genistein, Ornithine decarboxylase
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